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2 edition of Growth measurements of the chemolithotrophic bacterium thiobacillus ferrooxidans found in the catalog.

Growth measurements of the chemolithotrophic bacterium thiobacillus ferrooxidans

Allison J. Meldrum

Growth measurements of the chemolithotrophic bacterium thiobacillus ferrooxidans

by Allison J. Meldrum

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  • 24 Currently reading

Published by Laurentian University, Department of Biology in Sudbury, Ont .
Written in English


Edition Notes

StatementAllison J. Meldrum.
The Physical Object
Paginationvi, 25 l. :
Number of Pages25
ID Numbers
Open LibraryOL20635480M

Thiobacillus is a genus of Gram-negative Betaproteobacteria. Thiobacilus thioparus is the type species of the genus, and the type strain thereof is the Starkey T strain, isolated by Robert Starkey in the s from a field at Rutgers University in the United States of over 30 "species" have been named in this genus since it was defined by Martinus Beijerinck in , (the first. Respirometric experiments demonstrated that the oxygen uptake by Thiobacillus ferrooxidans strain LR was not inhibited in the presence of mM copper. Copper-treated and untreated cells from this T. ferrooxidans strain were used in growth experiments in the presence of cadmium, copper, nickel and zinc. Growth in the presence of copper was improved by the copper-treated cells.

Thiosulfate dehydrogenase is known to play a significant role in thiosulfate oxidation in the acidophilic, obligately chemolithoautotroph, Acidithiobacillus ferrooxidans. Enzyme activity measured using ferricyanide as the electron acceptor was detected in cell extracts of A. ferrooxidans ATCC grown on tetrathionate or sulfur, but no activity was detected in ferrous iron-grown cells. It is clear that iron metabolizing acidophilic microorganisms comprise a diverse range of prokaryotes that vary considerably in aspects of their physiologies, phylogenies, and biochemistries. Acidophilic ferrous iron-oxidizing prokaryotes have frequently been categorized on the basis of their temperature optima for growth. Three groups have been described: mesophilic iron oxidizers, which have.

Measurement of cell growth. Culture growth was moni-tored spectrophotometrically by reading optical density measurements at nm (OD) with a Beckman DU spectrophotometer. Culturesamples(1 ml) wereremovedat timed intervals and transferred to ml microfuge tubes, andthe cells wereharvested bycentrifugation at 11, x g for 10 minin a. Linear extrachromosomal DNA of Thiobacillus cuprinus DSM in relation to its chemolithotrophic growth Article in FEMS Microbiology Letters (1) - 78 January with 4 Reads.


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Growth measurements of the chemolithotrophic bacterium thiobacillus ferrooxidans by Allison J. Meldrum Download PDF EPUB FB2

Bhupinder Dhir, in Transgenic Plant Technology for Remediation of Toxic Metals and Metalloids, Other Approaches for Remediation of Hg. Enzymes such as cytochrome c oxidase from iron (Fe 2+)-oxidizing Hg-sensitive acidophilic bacterium Thiobacillus ferrooxidans also play a role in reduction of Hg 2+ (Iwahori et al., ).Mercury sensitive dissimilatory metal reducing bacteria.

D.B. Johnson, in Encyclopedia of Microbiology (Third Edition), Primary Producers in Acidic Environments. The first extremely acidophilic microorganism to be isolated and characterized was the sulfur-oxidizing bacterium, Acidithiobacillus (At.) thiooxidans (then referred to as Thiobacillus thiooxidans) by Waksman and Joffe in Some years later, another sulfur-oxidizing bacterium was.

First evidence for existence of an uphill electron transfer through the bc1 and NADH-Q oxidoreductase complexes of the acidophilic obligate chemolithotrophic ferrous ion-oxidizing bacterium Thiobacillus ferrooxidans.

Journal of Bacteriology– INTRODUCTION. Acidithiobacillus ferrooxidans is an acidophilic, chemolithoautotrophic bacterium that derives energy from the oxidation of ferrous iron and reduced inorganic sulfur compounds (RISCs). This bacterium is one of the key organisms used in industrial bioleaching applications (1, 2).The oxidation of ferrous iron by this bacterium has been previously examined in great detail (3–6).Cited by: The effects of naturally-occurring organic compounds on ferrous iron oxidation by the bacterium Thiobacillus ferrooxidans were examined with a view to using these compounds to treat or prevent acid mine/rock drainage.

The compounds glucose, cellobiose, galacturonic acid, and citric acid were added to the growth medium of five different strains of the bacterium and growth studies were done to.

1. Introduction. Thiobacillus Ferrooxidans is a gram negative, chemolithotrophic bacteria. Its ability to oxidize Fe +2 to Fe +3 ions and elemental sulfur in acidic solution is well known. Although bioleaching of low-grade ores is a well established application of bacteria in mineral processing, the use of bacteria in flotation is relatively new and few studies have been reported in the.

The most-studied leaching bacteria are from the genus Acidithiobacillus. Acidithiobacillus ferrooxidans and A. thiooxidans are acidophilic mesophiles and together with the moderate thermophile, A.

caldus, they belong to the Gram-negative γ-proteobacteria [25].The A. ferrooxidans has been considered as a model biomining microorganism [17, 22] and its cells can be seen by scanning electron. Bacterial strains and growth conditions. Acidithiobacillus ferrooxidans strain ATCC was grown in batch cultures by using thiosulfate-containing DSMZ medium 71 [in grams per liter: KH 2 PO 4, ; MgSO 4 7H 2 O, ; (NH 4) 2 SO 4, ; CaCl 2 2H 2 O, ; Na 2 S 2 O 3 5H 2 O, ; pH –].

No ferrous sulfate was added to the medium according to the instructions given by. Paiment A, Leduc LG, Ferroni GD. The effect of the facultative chemolithotrophic bacterium Thiobacillus acidophilus on the leaching of low-grade Cu-Ni sulfide ore by Thiobacillus ferrooxidans.

Geomicrobiol. 18 (2): Crossref, ISI, Google Scholar. Anaerobic Growth of Thiobacillus ferrooxidans Article (PDF Available) in Applied and Environmental Microbiology 58(7) August with Reads How we measure 'reads'.

The genome of Thiobacillus ferrooxidans ATCC is about X 10(6) base pairs as determined by analysis of reassociation kinetics of sheared DNA.

This is 70% of the size of the genome of. Some kinds of chemolithotrophic bacteria gain energy from inorganic matter and fix carbon dioxide from the air. One of them, the bacterium Thiobacillus ferrooxidans, "eats" metals.

Eccleston, M., Kelly, D. Oxidation kinetics and chemo-stat growth kinetics of Thiobacillus ferrooxidans on tetrathio-nate and thiosulfate. Journal of. The most-studied leaching bacteria are from the genus hiobacillus ferrooxidans and A.

thiooxidans are acidophilic mesophiles and together with the moderate thermophile, A. caldus, they belong to the Gram-negative γ-proteobacteria [25].The A. ferrooxidans has been considered as a model biomining microorganism [17, 22] and its cells can be seen by scanning electron.

The utility of such measurements for the study of the interactions of chemolithotrophic bacteria with their insoluble substrates is discussed. Tuovinen OH, Kelly DP. Studies on the growth of Thiobacillus ferrooxidans. Use of membrane filters and ferrous iron agar to determine viable numbers, and comparison with 14 CO 2 -fixation and iron.

Acidithiobacillus thiooxidans, formerly known as Thiobacillus thiooxidans until its reclassification into the newly designated genus Acidithiobacillus of the Acidithiobacillia subclass of Proteobacteria, is a Gram-negative, rod-shaped bacterium that uses sulfur as its primary energy source.

It is mesophilic, with a temperature optimum of 28 °C. This bacterium is commonly found in soil, sewer. Acidithiobacillus ferrooxidans is one of the main acidophilic chemolithotrophic bacteria involved in the bioleaching of metal sulfide ores.

The bacterium-mineral interaction requires the development of biofilms, whose formation is regulated in many microorganisms by type AI-1 quorum sensing.

The utility of such measurements for the study of the interactions of chemolithotrophic bacteria with their insoluble substrates is discussed. Discover the world's research 17+ million members.

A novel strain of an iron- and sulfur-oxidizing bacterium was isolated from a natural biotope at Kashen copper ore (Martakert Province, Republic of Artsakh).

The strain is able to grow and oxidize ferrous ions in the range of pH – with optimal pH The optimal temperature for growth is 35°C. aquatic food web. In the present study, a chemolithotrophic, acidophilic, iron-oxidizing, gram-negative, stricked shaped bacteria i.e Thiobacillus ferrooxidans preferring temperature range °C was isolated and microbiological characterization was performed.

The study revealed that it is. Introduction. Acidithiobacillus ferrooxidans is a Gram-negative, rod-shaped, acidophilic chemolithoautotrophic bacterium that uses CO 2 as a carbon source and obtains its energy for growth from by oxidizing ferrous iron, sulfur, and reduced sulfur compounds [].It is used widely in a variety of industrial processes, such as the bioleaching of metals, desulphurization of coal and natural gas.ofthe interactions ofchemolithotrophic bacteria with their insoluble substrates is discussed.

Thiobacillus ferrooxidans is the most extensively character-ized member of a group of chemolithotrophic bacteria that inhabit ore-bearing geological formations exposed to the at-mosphere and obtain all of their energy for growth from the.Thiobacillus and even lower. The intensive sulfuric acid produc- tion leads to a rapid decomposition of rocks so that The bacteria most active in bioleaching belong to acid-soluble metal compounds can pass into solution the genus Soljbilization.

These are Gram-negative, as sulfates. Morphologically the presence of yeast extract [13].